Congratulations to Jimin Wang and his recent publication in PNAS: “Cryo-EM meets crystallography: a model-independent view of the heteronuclear Mn4Ca cluster structure of photosystem II”!

Comparison with cryo-EM-derived CD map and XFEL-derived omit ED maps for metal cluster of 2F PSII. (A) The emd50019 derived CD map without any map sharpening contoured at 1.3σ is superimposed on its 9evx model. (B) The OEC-omit σA-weight 2Fo−Fc map contoured at 1.0 σ is superimposed onto the single-conformation model of the 6jll/2F dataset. (C) Stereodiagram of superposition of the two maps with the 9evx model. (D) Stereodiagram of superposition of the two atomic models. (E and F) Thin slice of 1B and in a 45°-rotated view. (G) At reduced 0.5σ contouring and thin slice of 1B.

March 21, 2025

Read more here: https://www.pnas.org/doi/10.1073/pnas.2423012122.